FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI13.H7LYJBBXX.s_2.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI13.H7LYJBBXX.s_2.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13531188
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG389970.2882008586385763No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA277180.20484528039962197No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC259860.1920452217499306No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT257580.19036022557664561No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT189780.14025376042369672No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT187470.13854659324813165No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA186930.13814751520709045No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT183320.1354796045993892No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT176660.13055764209321458No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG171950.12707679473524422No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA158230.11693725635916077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGA12750.023.142621
GGACCTT90650.021.33229643
TCGTGAG14350.021.3172862
TTATCCG58500.020.90848537
TATCCGA59250.020.60669338
GACCTTG100200.019.4308644
ATGGACC102000.019.23858841
ATCCGAG64750.018.92426739
AATGGAC106200.018.29130440
TGGACCT108500.018.1469442
CCCGTAT6500.017.9547941
GTTCGTT8900.017.5665421
CGTTGAT75050.017.5162681
TCCGAGT70900.017.34499240
CGCCGTT4700.017.3349171
GTCGTTT14100.017.1787471
GCCTATA72400.017.04602610
TGCCGAA23200.016.87907244
GGTTATC73350.016.82544135
CCCGTTT22500.016.7352241
Produced by FastQC (version 0.11.5)