FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI13.H7LYJBBXX.s_3.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI13.H7LYJBBXX.s_3.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13510826
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG369300.2733363600419397No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA268800.19895156669177738No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT251220.18593977895948036No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC251070.18582875687985323No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT185040.13695683742800033No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT180250.133411532351908No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT180150.13333751763215662No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA179090.13255296160279173No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT172760.12786782984252776No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG163920.12132492861650353No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA154440.11430833318407031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCCG54900.021.4794837
TATCCGA55000.021.44042838
GGACCTT88700.020.71074943
GTCGCGA1401.8553692E-1020.43451
GTCGTGA11700.019.9374071
GACCTTG95250.019.40131444
ATCCGAG61400.019.3131639
ATGGACC101400.018.16019241
GTCGGGT30650.017.8779431
TCCGAGT66500.017.86508440
AATGGAC104350.017.62571540
GGTTATC66850.017.57330735
TGGACCT106500.017.24923542
GTCGTTT13450.017.1797051
GTTCGTT9050.017.0215131
GCCTATA71750.016.86363610
CCCGCTA6550.016.7987671
CGTTGAT79800.016.6013071
TGCCGAA20500.016.5266144
CGCGTAA953.3420726E-416.215231
Produced by FastQC (version 0.11.5)