FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI13.HC332BBXX.s_1.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI13.HC332BBXX.s_1.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13538040
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG380400.2809860216102183No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA280630.20728997698337423No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC261470.19313726359207092No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT260860.19268668138076117No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT189750.14016061409184785No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA186710.1379150896289271No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT185780.1372281364215204No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT182300.13465760183896636No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT176820.1306097485308065No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG170370.1258453956407279No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA154400.11404900561676579No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT136040.10048721971570478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCCG58350.022.13122637
TATCCGA59300.021.88684338
GTCGTGA11450.021.369741
GGACCTT89600.020.52610443
ATCCGAG64650.020.10966139
TCCGAGT68150.019.30383540
GACCTTG95700.019.2406744
CCCGCTA6150.018.996891
ATGGACC100400.018.62487841
GGTTATC72950.018.00344835
GCCTATA73750.017.92760710
GTCGGGT29150.017.7709661
CGCCGTT4850.017.7257521
GTTCGTT10700.017.511241
CCCGATT9000.017.389921
AATGGAC106950.017.29909940
TGGACCT107450.017.28002242
TATTGCC77850.017.2659656
CGTTGAT81200.017.2113511
CCCGTTT18950.017.0997541
Produced by FastQC (version 0.11.5)