FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI13.HC3LTBBXX.s_2.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI13.HC3LTBBXX.s_2.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13334852
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG364790.2735613413632187No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA275490.20659396894693693No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT264810.19858488118203338No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC259890.19489530142516767No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT187140.14033901538614751No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA183720.137774307506375No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT181130.13583202873192743No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT176850.13262239430928816No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT175030.13125754976508175No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG157440.11806655221970218No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA148370.11126482693621195No Hit
GTGTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGC134790.10108098687559489No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT133490.10010609791544743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGA11400.021.4335981
TTATCCG55950.021.1939337
TATCCGA55850.021.07431438
GGACCTT84850.020.71563343
GACCTTG91700.019.1680344
ATCCGAG61950.018.999239
CGCCGTT4250.018.6462081
ATGGACC96600.018.51525541
GTCGTTT12650.018.2715641
CGGCTAT6050.018.1924671
GTCGGGT27500.018.0105421
TCCGAGT68000.017.40588840
GGTTATC68650.017.2731335
AATGGAC102200.017.26419440
TGGACCT103550.017.08084142
CCCGATT8450.016.9329871
CCCGAAT11050.016.7337761
CGTTGAT76050.016.730371
GCCTATA72250.016.44260410
CCCGTCT14600.016.4342771
Produced by FastQC (version 0.11.5)