FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI14.H7LYJBBXX.s_3.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI14.H7LYJBBXX.s_3.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12296551
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG405700.3299299128674374No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA265540.21594673172989726No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT242320.19706338793699144No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC232590.1891506000341071No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA215950.1756183502186914No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT173700.14125912217173742No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT170850.13894139909638076No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT161850.13162227359525447No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT154080.12530342857928212No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG153200.12458778075250532No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT144800.11775659695145411No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA131740.10713573261315307No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA125280.10188222697567798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT89400.021.65588643
GACCTTG92850.020.99287844
TATCCGA57850.020.38388338
TTATCCG58450.020.36283537
GTCGTGA9150.019.9628281
ATGGACC97750.019.94103841
AATGGAC98650.019.7368140
GTTCGTT9350.019.0650711
GTCGTTT12850.019.010121
TGGACCT103400.018.957842
ATCCGAG62350.018.94822939
TCCGAGT66500.017.93116240
CCCGTCT14700.017.8153611
CAATGGA110000.017.80033339
CCCGTTT18550.017.6769541
GTACGTA58150.017.5603561
CGTTGAT75400.017.1621231
CCCGCTA6450.017.0598551
GGTTATC69950.017.01477435
CCTACGT114800.016.97837425
Produced by FastQC (version 0.11.5)