FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI15.H7LYJBBXX.s_1.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI15.H7LYJBBXX.s_1.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11381312
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG330920.2907573397513397No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT311850.27400180225267523No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA272740.23963845293055844No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC218970.1923943390709261No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT188030.16520942401016683No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT183510.1612380013833203No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT182660.16049116305747527No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT166480.1462748758666839No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA160800.14128423858339004No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT149600.13144354534872604No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA146140.1284034740458745No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG144070.1265847030641107No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA143420.1260135914031704No Hit
GTGTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGC140710.1236324950937115No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA130150.11435412718674262No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT119620.10510211827950942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCGA68250.022.65794838
TTATCCG68500.022.5398737
CCCGTAT6700.022.3692991
GTTCGTT9400.021.8058661
GGACCTT77700.021.33472643
ATCCGAG73300.021.12693839
GTCGTGA10700.020.804431
TGCCGAA22700.020.62728744
GACCTTG81650.020.19380644
TCCGAGT77800.020.07458740
CCCGTTT18150.019.9152371
GGTTATC80550.019.3583535
ATGGACC86950.019.27803841
CCCGAAT10900.018.6028541
GCCTATA85850.018.30955910
AATGGAC91250.018.2968740
TGGACCT93700.017.95970742
TATTGCC88850.017.6918856
TACTAAC50750.017.50739728
TGGTTAT89550.017.48800534
Produced by FastQC (version 0.11.5)