FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI15.H7LYJBBXX.s_3.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI15.H7LYJBBXX.s_3.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11099628
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG320770.2889916671081229No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT297740.2682432240071469No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA261990.2360349373870908No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC207510.18695221137140813No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT179330.16156397313495552No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT178920.16119459138630593No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT177320.15975310163547823No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT158500.1427975784413676No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA153260.13807669950740692No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT142880.1287250347489123No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA142180.1280943829829252No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA138320.12461678895905341No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG138320.12461678895905341No Hit
GTGTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGC136280.1227788895267481No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA124700.11234610745513272No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT113570.10231874437593766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCCG63000.023.25758637
TATCCGA63650.022.91638438
TGCCGAA21350.021.94820244
GGACCTT73600.021.94073743
ATCCGAG69000.021.1398239
GTCGTTT12500.020.4218161
GTCGTGA9700.020.1913171
GACCTTG80050.020.1721444
TCCGAGT73900.019.88698240
GGTTATC74550.019.7716335
ATGGACC86300.019.06881541
CGCCGTT4550.018.8625141
AATGGAC88550.018.46022840
ATGCCGA25700.018.40510243
GCCTATA81200.018.28753910
GTTCGTT9900.018.2274131
CCCGGTT11600.018.2120841
TGGACCT90500.018.20816442
CTCGCGT2100.017.8145961
TGGTTAT83150.017.72669834
Produced by FastQC (version 0.11.5)