FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI16.H7LYJBBXX.s_3.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI16.H7LYJBBXX.s_3.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12312492
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG774520.6290521853740088No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT356000.2891372437033868No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG349420.2837930777944871No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT332160.26977479457448583No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA315030.2558620951794324No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA219040.17790062320446584No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT213190.17314935108181187No Hit
GTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCTG211930.17212600016308638No Hit
CGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCGGG197540.16043868292462649No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC196890.15991076379988714No Hit
GCCGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCG187580.15234933756708227No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT183760.14924679748015268No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC172370.13999603004818195No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA165620.13451379298358124No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGC158510.12873916994220178No Hit
CTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATA158500.1287310481095135No Hit
GTGACTTGCGGGGGAAGTCTACGCGCGTGTGCACGCGGCACTCTCTGCCC151910.12337876036792551No Hit
CAGTGACTTGCGGGGGAAGTCTACGCGCGTGTGCACGCGGCACTCTCTGC147290.11962647366593213No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC146550.11902545804699813No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG146200.11874119390290772No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT146080.11864373191064816No Hit
CCCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAG145060.11781530497644183No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG135110.1097340814515859No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA129460.10514524598269791No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA126600.10282240183384485No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC126270.10255438135513104No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT124480.10110057330392579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT148500.025.3924243
GTCGTGA13950.025.2407991
GACCTTG156650.024.22532344
CGGCTAT8500.022.7835331
TCGTGAG15400.021.8609892
ATGGACC179450.021.14780841
AATGGAC181200.020.91936740
TGGACCT183950.020.5108742
GTCGTTT15250.020.4915961
ACTGCGT28800.020.1668576
TGCCGAA38250.019.67005544
CAATGGA194600.019.55801239
CCCGCTA10150.019.5134371
GTCGCGT1252.2848326E-819.3660031
TTATCCG27750.019.02697437
TATCCGA29250.018.12644638
GACTGCG32750.017.8016955
TACAATG219650.017.36758237
TCCTACA223600.017.08010734
CCCGTAT7150.016.9283241
Produced by FastQC (version 0.11.5)