FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI16.HC3LTBBXX.s_2.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI16.HC3LTBBXX.s_2.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12126312
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG759790.6265631298287558No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT346890.2860638914782994No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG331370.27326527636762105No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT325490.2684163165189878No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA307410.2535065896374759No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT213200.17581602716473071No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA208710.1721133350354172No Hit
GTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCTG208460.1719071717765467No Hit
CGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCGGG192920.15909206360515876No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC190840.1573767852913565No Hit
GCCGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCG186100.15346792990317254No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT182590.15057339774863124No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC170080.14025698827475327No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA159500.1315321591593553No Hit
CTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATA151960.12531427527182212No Hit
GTGACTTGCGGGGGAAGTCTACGCGCGTGTGCACGCGGCACTCTCTGCCC151490.12492668834514567No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGC150290.12393710470256743No Hit
CAGTGACTTGCGGGGGAAGTCTACGCGCGTGTGCACGCGGCACTCTCTGC146090.12047356195354365No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG145900.1203168778768021No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC143850.11862633915406431No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT143290.11816453345419448No Hit
CCCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAG139910.11537720619426582No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG132050.10889543333537847No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC130420.10755124888754307No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA129100.10646270688070701No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA124300.10250437231039414No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT122090.10068188910197923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT144300.025.50550343
GACCTTG153500.024.06262644
GTCGTGA16250.022.7589471
CCCGCTA10000.022.4541381
ATGGACC179550.020.69521541
TGCCGAA36600.020.4962344
AATGGAC181800.020.28193940
TCGTGAG17350.020.0391032
GTCGTTT15800.019.9239371
TGGACCT184450.019.91783142
ACTGCGT25550.019.71726
CGGCTAT8700.019.4835321
CAATGGA196700.018.79032339
TTATCCG29150.018.71726837
GACTGCG27600.018.4918175
TATCCGA30650.017.8012538
TCCTACA217750.017.05383734
TACAATG217850.016.9660637
CTACGAG8750.016.84492915
TCTCCTA222600.016.682232
Produced by FastQC (version 0.11.5)