FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI18.H7LYJBBXX.s_2.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI18.H7LYJBBXX.s_2.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15336570
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG1588901.0360204400332018No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT564090.3678071433182256No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG555340.36210182589718565No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT544940.3553206486196066No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA466820.3043835746845611No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA402850.26267281406468335No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC383240.24988638267878674No Hit
GTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCTG381290.24861491193924065No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT374820.24439623722905449No Hit
CTGAGAATGAGCCCCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCG329510.21485247353221745No Hit
CGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCGGG326510.21289636470214657No Hit
GTGATCCACCGCTAAGAGTCGAGCGACGCTCAGACAGGCGTAGCCCCGGG325480.2122247673371556No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG316310.2062455946799056No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC290460.18939045692746162No Hit
CTCAGACTGCGTTCCCTCCCTCTCACCCTCCCAAAGGAGGGAAGAACGAT278390.18152037906780982No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT277730.18109003512519423No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG266930.1740480433369391No Hit
GTGATCCACCGCTAAGAGTCAGCGACGCTCAGACAGGCGTAGCCCCGGGA265100.17285481695059587No Hit
CTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATA263260.17165507020148574No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGC258660.16865570332871038No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA257640.1679906263264863No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC249100.16242223652355123No Hit
GTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCTGACGGA243100.15851001886340949No Hit
CCCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAG231380.15086815370059928No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA225150.14680596769681878No Hit
GTCTGAATTGGGTTATGAGGCCCGGGAGGTGCCTCACCTCAGCCATTGAA210720.1373970842241779No Hit
GCCGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCG208940.13623645965166917No Hit
GAGTGATCCACCGCTAAGAGTCGAGCGACGCTCAGACAGGCGTAGCCCCG208660.1360538894941959No Hit
GTGACTTGCGGGGGAAGTCTACGCGCGTGTGCACGCGGCACTCTCTGCCC207390.13522580342279922No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC204260.13318492987675862No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA198770.12960525071772894No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT197040.12847722795905472No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGCTT189370.1234761097168402No Hit
GCCGAAGATAATTAGTTTGGGTTAATCGTATGACCGCGGTGGCTGGCACG185390.12088100533561286No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT182430.1189509779566096No Hit
CTGCGTTCCCTCCCTCTCACCCTCCCAAAGGAGGGAAGAACGATCATCAA182410.11893793723107579No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA181230.11816853442458125No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC178800.11658408627222384No Hit
CAGTGACTTGCGGGGGAAGTCTACGCGCGTGTGCACGCGGCACTCTCTGC177840.11595813144660116No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT174820.11398898189099649No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC168430.10982247008294553No Hit
CTCGTATACCCTTGACCGAAGACCGGTCCTCCTCTATTCGGGGAAGGTCG166790.10875313058917345No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG166650.10866184551043681No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA166000.10823802193058814No Hit
CGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCT165050.10761858746773235No Hit
CCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGC164950.10755338384006333No Hit
CCGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCGG156310.10191979040945923No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT155270.10124167268170131No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC154970.10104606179869424No Hit
GAGTGATCCACCGCTAAGAGTCAGCGACGCTCAGACAGGCGTAGCCCCGG153800.10028317935496658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT279350.027.88695343
GACCTTG287300.027.09996444
ACTGCGT50750.025.2718186
CCCGCTA12600.024.4650081
GTCGTGA14950.024.4486431
GACTGCG52700.024.3792515
ATGGACC330900.023.70139141
GGGTGAT61350.023.4720521
TGGACCT334000.023.42220542
AATGGAC335250.023.36760940
CGGCTAT10600.023.2648371
CAATGGA350450.022.35372539
TCGTGAG16850.021.9412142
GTCGTTT16800.020.9700071
TCCTACA377500.020.75168234
TACAATG382050.020.58542437
GTACGTA44500.020.4846321
TCTCCTA391750.020.1316132
GGTGATC82650.020.1293722
CCTACAA391650.020.05275735
Produced by FastQC (version 0.11.5)