FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI19.H7LYJBBXX.s_1.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI19.H7LYJBBXX.s_1.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13611065
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG966420.7100252625345629No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT390010.2865389299073952No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT360500.26485804013131964No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG356770.26211762268419114No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA332770.24448490988765392No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT308900.2269477076187646No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA304100.22342116505945717No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC264380.194239025381188No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT236170.17351324088159154No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA233260.17137527445501144No Hit
GTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCTG225090.16537280514052355No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA222470.1634479006602349No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT215690.1584666592952131No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC213500.15685767425252908No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGC209460.15388950093177867No Hit
CGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCGGG205640.15108296081166314No Hit
CTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATA204110.1499588753708839No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT195730.14380211981942634No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT190900.14025353636912322No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT182900.13437596543694413No Hit
GCCGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCG175010.12857921110508252No Hit
CCCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAG173130.12719798193602044No Hit
CTCAGACTGCGTTCCCTCCCTCTCACCCTCCCAAAGGAGGGAAGAACGAT165880.12187143327873314No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC156740.11515630848871856No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC153860.11304038295313408No Hit
GTCTGAATTGGGTTATGAGGCCCGGGAGGTGCCTCACCTCAGCCATTGAA153210.11256283031489454No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG152230.1118428278757026No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG149190.10960935092147454No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA148280.10894077722793918No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA146510.10764036465919456No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA146030.1072877104032638No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCG145670.10702321971131576No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA144970.10650893225475008No Hit
CTGAGAATGAGCCCCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCG144520.10617831888981501No Hit
ATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCTCCATAG141170.10371708606196502No Hit
CAGTGACTTGCGGGGGAAGTCTACGCGCGTGTGCACGCGGCACTCTCTGC140960.10356279982499533No Hit
CGGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTA140880.10350402411567353No Hit
GTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAATACTTGTAATGCTA139940.1028134095311425No Hit
GTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTA137270.10085177023252774No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT137060.10069748399555803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT175250.027.22213643
GACCTTG184400.025.82303844
CCCGCTA12700.024.9902251
GTCGTGA14000.022.984561
ATGGACC215250.022.39156741
TGGACCT218500.021.93769842
AATGGAC220100.021.77710340
ACTGCGT36400.021.3210226
TTATCCG69600.020.60363237
GACTGCG37850.020.5042345
CAATGGA234400.020.4860939
TCGTGAG15350.020.33832
TATCCGA70750.020.1466838
CGCCGTT7800.019.4969141
CCCGTAT6900.019.4846291
GTCGTTT14150.018.8469161
TCCTACA258700.018.534834
TGCCGAA36650.018.34455744
ATCCGAG78750.018.10036739
TCTCCTA270200.017.89246432
Produced by FastQC (version 0.11.5)