FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI20.HC3LTBBXX.s_2.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI20.HC3LTBBXX.s_2.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9172229
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG480700.5240819870502579No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT196960.21473515325445974No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT158250.1725316714181471No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG146540.15976487285696858No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA141350.15410648818297057No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA138020.1504759639123707No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC117440.12803866977154627No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT116360.12686120244054092No Hit
GTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCTG115550.12597810194228687No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT107480.1171798043856079No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA103830.11320040090582126No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA98980.10791270039158421No Hit
CGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCGGG91840.1001283330366043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT93200.024.78506743
GACCTTG100200.023.18455344
AATGGAC110350.020.99337440
ATGGACC111150.020.96091841
GTCGTTT11000.020.211291
TGGACCT114950.020.0571442
CAATGGA119150.019.55366139
CCCGCTA5300.019.5203381
GTCGTGA8950.019.429851
TGCCGAA19050.018.59231244
TTATCCG26500.018.18127837
CGCCGTT5050.017.8713681
TATCCGA27200.017.71337738
TCCTACA132450.017.62310834
ACTGCGT13000.017.4300546
CCTACGT136050.017.1561325
TACGTTC136100.017.13366327
CTCCTAC136550.017.07747833
CTACGTT137200.017.04439726
TCTCCTA138400.017.0399532
Produced by FastQC (version 0.11.5)