FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI21.H7LYJBBXX.s_3.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI21.H7LYJBBXX.s_3.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7519709
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG224750.298881246601431No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA173910.2312722473702107No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT137900.18338475597925397No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC127070.16898260291721395No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA121850.16204084493163234No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT114330.1520404579485722No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT98940.13157424043935745No Hit
CTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGC95400.12686661146062966No Hit
GTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAATACTTGTAATGCTA91240.12133448249127725No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT91120.12117490184793056No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT86570.11512413578770135No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA84670.11259744226804522No Hit
CGGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTA83320.11080216003039478No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT82940.11029682132646357No Hit
ATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCTCCATAG79350.10552270041300801No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG78460.10433914397485328No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA77950.10366092624062978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA502.3575249E-630.8101961
GGACCTT51800.021.83051743
ATGGACC54000.021.0633541
AATGGAC56100.020.4317540
GACCTTG57500.020.23970244
TTATCCG31200.019.9552337
TGGACCT58900.019.53516442
TATCCGA31650.019.53248638
CCTACGT60950.018.62445425
TACGTTC61350.018.610627
CCCGTAT4550.018.379711
CTACGTT62600.018.30926926
GTACGTA33550.018.0387571
ATCCGAG34700.018.00609639
GACTACC63300.018.00313419
CAATGGA63400.017.90569339
CCCTACG63550.017.86247824
ACTACCC64250.017.73693820
CTCGTTT62550.017.6269361
ACCCTAC64750.017.59938823
Produced by FastQC (version 0.11.5)