FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI22.H7LYJBBXX.s_1.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI22.H7LYJBBXX.s_1.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19510063
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG1948320.9986231207966884No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT815020.417743397343207No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG691270.35431459139829535No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT639680.32787182696437217No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA590140.3024798023461021No Hit
GTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCTG417600.21404338878864715No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA415420.21292601669200148No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT414390.21239808400413673No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC410450.2103786133340523No Hit
CTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATA404340.20724689612739844No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGC393280.20157802668294816No Hit
GTCTGAATTGGGTTATGAGGCCCGGGAGGTGCCTCACCTCAGCCATTGAA380380.19496605418444832No Hit
CGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCGGG377180.19332587496001422No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT369240.18925618025938717No Hit
GCCGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCG335160.17178827151916426No Hit
CAGTGACTTGCGGGGGAAGTCTACGCGCGTGTGCACGCGGCACTCTCTGC329000.16863092651212863No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC314390.16114248324057182No Hit
GTGACTTGCGGGGGAAGTCTACGCGCGTGTGCACGCGGCACTCTCTGCCC303160.15538647927482346No Hit
CCCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAG300440.1539923269340545No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG289360.1483132063694515No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC286440.1468165428271554No Hit
CTGAGAATGAGCCCCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCG285270.1462168522982217No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA275190.14105028774125436No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG274710.14080426085758924No Hit
CTCAGACTGCGTTCCCTCCCTCTCACCCTCCCAAAGGAGGGAAGAACGAT273960.1404198438518625No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCG270500.13864640006544315No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC269570.138169722978342No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA264010.13531991157588777No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT263220.13491499232985563No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA262250.13441781300244904No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT250270.12827739203097396No Hit
CTCGTATACCCTTGACCGAAGACCGGTCCTCCTCTATTCGGGGAAGGTCG236670.12130663032712914No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA232120.11897450049238693No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG224750.1151969627161122No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT221040.11329537992778393No Hit
GTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCTGACGGA219810.11266493603839207No Hit
CCGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCGG218800.11214725447068008No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC210580.10793404408791504No Hit
CCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGC210450.1078674118069224No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCAAA208480.10685767647188019No Hit
GGGGAGAGTAGTCTGAATTGGGTTATGAGGCCCGGGAGGTGCCTCACCTC208270.1067500397102767No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGCTT207760.10648863614638251No Hit
CTGCGTTCCCTCCCTCTCACCCTCCCAAAGGAGGGAAGAACGATCATCAA197780.10137332719017873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT341100.028.0378443
GTCGTGA22750.027.5138511
GACCTTG351650.027.18383244
CCCGCTA21250.024.0626491
TCGTGAG24450.024.0075572
CGCGTAA1153.43789E-1022.9984111
ATGGACC426850.022.55806541
CGGCTAT13250.022.4559961
TGGACCT431600.022.2231542
AATGGAC432650.022.1631140
ACTGCGT57300.021.9843376
CAATGGA458700.020.91403839
GACTGCG61650.020.3974695
TGCCGAA65700.019.21730844
TACAATG504750.019.04756237
TCCTACA509050.018.89637434
TTATCCG44500.018.7827237
GTCGTTT21000.018.5766931
TCTCCTA527550.018.29626332
CCTACAA526050.018.28782835
Produced by FastQC (version 0.11.5)