FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI22.H7LYJBBXX.s_2.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI22.H7LYJBBXX.s_2.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18951980
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG1912821.0092982369124492No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT795080.419523448209633No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG707640.37338578871442457No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT641540.3385081664290486No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA586010.30920779781321No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC420610.22193459469670188No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA413410.21813551934942946No Hit
GTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCTG412750.21778727077592946No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGC405930.21418870218309644No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT401540.21187232151996782No Hit
CTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATA399860.21098587060560425No Hit
CGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCGGG373940.19730919935542357No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT370670.19558378596853734No Hit
GTCTGAATTGGGTTATGAGGCCCGGGAGGTGCCTCACCTCAGCCATTGAA366390.1933254467343254No Hit
GCCGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCG334760.1766358976740161No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC316440.16696936151262295No Hit
CAGTGACTTGCGGGGGAAGTCTACGCGCGTGTGCACGCGGCACTCTCTGC309040.16306475629459297No Hit
CCCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAG297580.15701789470018437No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC288930.15245372778991956No Hit
GTGACTTGCGGGGGAAGTCTACGCGCGTGTGCACGCGGCACTCTCTGCCC288200.15206854376165446No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG286130.15097630959931363No Hit
CTGAGAATGAGCCCCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCG282710.14917174880935924No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCG280800.14816393854362447No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA278790.1471033633425109No Hit
CTCAGACTGCGTTCCCTCCCTCTCACCCTCCCAAAGGAGGGAAGAACGAT278250.1468184326914655No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG270570.1427660856543749No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA266550.14064493525214777No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC263370.13896701030710248No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA259270.13680364795657235No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT256140.1351521054792164No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT253990.13401765936857257No Hit
CTCGTATACCCTTGACCGAAGACCGGTCCTCCTCTATTCGGGGAAGGTCG239910.12658835646723984No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG226790.11966559694554342No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA224510.11846255641890714No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT222360.11732811030826332No Hit
GTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCTGACGGA220840.11652608329050579No Hit
CCGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCGG214040.11293806768474851No Hit
CCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGC209430.11050560416378657No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCAAA208750.11014680260321086No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC204450.10787791038192317No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGCTT201450.10629496232055964No Hit
CTGCGTTCCCTCCCTCTCACCCTCCCAAAGGAGGGAAGAACGATCATCAA201250.10618943244980207No Hit
GGGGAGAGTAGTCTGAATTGGGTTATGAGGCCCGGGAGGTGCCTCACCTC199570.10530298153543852No Hit
CGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCT195380.10309213074306749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT329650.027.7823543
GACCTTG338750.026.99705344
GTCGTGA23650.025.7891
CGGCTAT11100.023.4069541
CCCGCTA21300.023.1555081
ATGGACC412250.022.44476541
TCGTGAG27400.022.4089012
TGGACCT416500.022.15769442
AATGGAC416000.022.11023340
ACTGCGT56650.021.3973736
CAATGGA441250.020.88466639
CCCGTTT30000.020.2569351
GACTGCG62350.019.4418015
TGCCGAA61300.019.27211844
TACAATG480550.019.18579537
CGCCGTT8100.019.0282541
TCCTACA487750.018.88003234
CCGCTAC25950.018.8270632
TCTCCTA503550.018.35752932
CCTACAA504150.018.29645735
Produced by FastQC (version 0.11.5)