FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI24.H7LYJBBXX.s_1.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI24.H7LYJBBXX.s_1.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11897730
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG1481201.244943363145743No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT436660.3670111861674454No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT396150.33296267439251015No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG387080.32533937145993397No Hit
GTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCTG386660.32498636294486427No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA334130.2808350836672206No Hit
CGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCGGG303630.2551999415014461No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGC261050.21941160204509597No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC260290.21877282473211274No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA240860.20244197842781775No Hit
GTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCTGACGGA238940.20082822521607066No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA233550.1962979492726764No Hit
GCCGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCG233330.196113040050497No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA221040.18578333850238657No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT210550.17696653059028908No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC203900.1713772291016858No Hit
CTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATA199810.16793959856207866No Hit
CCCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAG195080.16396405028522248No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC192050.16141734599793406No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA160000.13447943431225956No Hit
CCGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCGG159080.13370617756496408No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC155770.13092413426762922No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCG155150.1304030264596692No Hit
CCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGC151670.12747809876337754No Hit
CGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCT149990.12606606470309883No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG148750.1250238490871788No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG147780.12420856751666075No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGCTT147330.1238303441076575No Hit
GTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCTGAC142750.11998087030046908No Hit
GTCTGAATTGGGTTATGAGGCCCGGGAGGTGCCTCACCTCAGCCATTGAA138680.11656004969015099No Hit
GCCGAAGATAATTAGTTTGGGTTAATCGTATGACCGCGGTGGCTGGCACG135220.11365193192314836No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT133180.11193731913566707No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA130410.10960914392913607No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT129450.10880226732326251No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT129050.10846606873748187No Hit
CTGAGAATGAGCCCCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCG128980.10840723398497024No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT123960.1041879417334231No Hit
GGGGAGAGTAGTCTGAATTGGGTTATGAGGCCCGGGAGGTGCCTCACCTC120270.10108650977959661No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT119910.10078393105239403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT239300.029.72000143
GACCTTG245550.028.94521744
GTCGTGA15600.026.2735671
ATGGACC282400.025.48200841
AATGGAC285300.025.17534640
TGGACCT287450.024.98075942
TCGTGAG16300.024.4123362
CAATGGA295250.024.3194839
TCCTACA297400.024.18530334
TGGAGTC302650.023.5645162
GGAGTCT305750.023.5125453
CTCCTAC306350.023.4859133
CCCGCTA8100.023.3961351
GAGTCTT308300.023.3109364
TCTCCTA311900.023.12441632
TACGTTC312750.022.99273527
CCTACGT313350.022.95562725
CCTACAA314800.022.88084235
CTACGTT315350.022.79619226
GACTACC316400.022.72092419
Produced by FastQC (version 0.11.5)