FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI24.HC3LTBBXX.s_2.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI24.HC3LTBBXX.s_2.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11362620
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG1369471.205241396790529No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT406420.3576815910415027No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT369270.3249866668074793No Hit
GTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCTG366960.32295368497758437No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG353470.3110814231224841No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA306200.26948010230034974No Hit
CGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCGGG281830.24803258403431602No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGC246060.216552168425944No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC245220.21581290230598227No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA229060.2015908302838606No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA225830.19874817603686476No Hit
GTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCTGACGGA223890.19704082333123876No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA217680.19157553451580708No Hit
GCCGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCG213080.18752717243030215No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT198150.17438759722669597No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC191560.16858787849985302No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC190210.16739977223562877No Hit
CTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATA182400.16052635747741278No Hit
CCCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAG181210.15947906380746696No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA146520.12894913321047435No Hit
CCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGC145450.12800744898623734No Hit
CCGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCGG145420.12798104662481014No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCG142990.12584245534920643No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC142950.12580725220063682No Hit
CGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCT139870.12309660976077699No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG139300.12259496489366009No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG138940.1222781365565336No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGCTT136970.12054438148948042No Hit
GCCGAAGATAATTAGTTTGGGTTAATCGTATGACCGCGGTGGCTGGCACG135810.11952349018096177No Hit
GTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCTGAC134240.11814176659960467No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT129210.11371497066697646No Hit
GTCTGAATTGGGTTATGAGGCCCGGGAGGTGCCTCACCTCAGCCATTGAA128870.11341574390413478No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT128840.11338934154270758No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT124420.10949939362576588No Hit
CTGAGAATGAGCCCCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCG122980.10823208027726No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA121360.10680635276019088No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT119600.10525741422312812No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT116340.10238835761470506No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT114590.1008482198647847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT216450.030.0744543
GACCTTG224400.029.01814344
GTCGTGA13350.028.0312061
ATGGACC255000.025.5201741
AATGGAC256300.025.4081240
TGGACCT258800.025.05956342
CAATGGA265850.024.4871239
TCCTACA269350.024.24975634
GGAGTCT276400.023.5966223
CTCCTAC277250.023.54280333
TGGAGTC273300.023.5423262
TCGTGAG15550.023.3488332
TCTCCTA280400.023.33324632
GAGTCTT281700.023.2855634
TACGTTC284850.022.94515427
CCTACGT285850.022.8723825
CTACGTT287300.022.75694326
CCCGCTA7450.022.7514231
GACTACC288550.022.61271519
ACCCTAC289950.022.59447923
Produced by FastQC (version 0.11.5)