FastQCFastQC Report
Wed 15 Jun 2016
SLX-11097.RPI26.HC332BBXX.s_1.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11097.RPI26.HC332BBXX.s_1.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15049485
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG2094791.3919346741765581No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT707120.4698632544568801No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG548900.3647300887704795No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT540880.3594010027585662No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA431460.2866941958478978No Hit
GTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCTG426390.28332530980296006No Hit
CGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCGGG392220.26062021391429674No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGC353090.2346193241828541No Hit
GCCGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCG346440.2302005683250955No Hit
CTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATA346180.23002780493817562No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC345260.22941648833830525No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA330690.21973509392514096No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC289500.19236538658964078No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT282820.1879266964949299No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC274980.18271721590473028No Hit
CCCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAG261830.1739793753739746No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA256340.17033141001170474No Hit
GTCTGAATTGGGTTATGAGGCCCGGGAGGTGCCTCACCTCAGCCATTGAA234800.15601862787995735No Hit
GTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCTGACGGA233580.15520796891056404No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCG232560.15453020485418603No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA229560.1525367811589566No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT227730.15132079270486665No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA225520.14985230391604762No Hit
CCGTGAGTCTGTTCCAAGCTCCGGCGAGGGAGGCATCCGCCCCGACTCGG223640.14860309173370384No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA207010.13755287971648197No Hit
CCGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGC205760.1367222865101364No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT203530.13524050823001585No Hit
GTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTA199720.13270886013707445No Hit
CTCGTATACCCTTGACCGAAGACCGGTCCTCCTCTATTCGGGGAAGGTCG190740.12674187854268767No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGCTT189440.12587806160808823No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC189090.12564549551031148No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG186700.1240574012997787No Hit
CGTGTGGTTGGTGCGAGCCAGGTCGCACTGCCTGCGTCACTATGTGAGCT182070.1209808840634746No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT180160.11971173764417853No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT174160.11572489025371965No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT173360.11519331060165845No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC169410.11256863606960636No Hit
GCCGAAGATAATTAGTTTGGGTTAATCGTATGACCGCGGTGGCTGGCACG167550.11133271337856411No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT165810.11017652763533105No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT165450.10993731679190351No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCAAA160920.10692724701210705No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG160840.10687408904690093No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT158280.10517303416030514No Hit
GCTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTT157040.10434908569961032No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT155680.10344540029110631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTT322900.030.3314543
GACCTTG332650.029.48862544
GTCGTGA20600.028.146811
AATGGAC399100.024.70564340
ATGGACC400600.024.6735441
TGGACCT404200.024.4592342
TCGTGAG22850.024.2606052
CAATGGA413650.023.76646639
CCCGCTA11700.023.5540071
TCCTACA421000.023.47850434
CTCCTAC431000.022.97459433
TCTCCTA436150.022.78401832
GGAGTCT445000.022.3294163
TGGAGTC439100.022.3138282
GAGTCTT446500.022.2699954
TACGTTC450700.021.95034627
CCTACGT451850.021.8944825
CCTACAA452350.021.82701735
GACTACC455050.021.82204419
TACCCTA455800.021.79074922
Produced by FastQC (version 0.11.5)