Alignment
| Sequences analysed in total |
40,817,621 |
| Single-end alignments with a unique best hit |
28,343,431 |
| Sequences without alignments under any condition |
4,070,690 |
| Sequences that did not map uniquely |
8,403,500 |
| Genomic sequence context not extractable (edges of chromosomes) |
6 |
Cytosine Methylation
| Total C's analysed |
252,330,135 |
| Methylated C's in CpG context |
3,133,999 |
| Methylated C's in CHG context |
2,194,953 |
| Methylated C's in CHH context |
7,170,938 |
| Methylated C's in Unknown context |
5 |
| Unmethylated C's in CpG context |
7,824,375 |
| Unmethylated C's in CHG context |
53,581,885 |
| Unmethylated C's in CHH context |
178,423,985 |
| Unmethylated C's in Unknown context |
37 |
| Percentage methylation (CpG context) |
28.6% |
| Percentage methylation (CHG context) |
3.9% |
| Percentage methylation (CHH context) |
3.9% |
| Methylated C's in Unknown context |
N/A% |
Alignment to Individual Bisulfite Strands
| OT |
14,364,802 |
original top strand |
| CTOT |
0 |
complementary to original top strand |
| CTOB |
0 |
complementary to original bottom strand |
| OB |
13,978,623 |
original bottom strand |
Deduplication
| Alignments analysed |
28,343,425 |
| Unique alignments |
22,388,069 |
| Duplicates removed |
5,955,356 |
| Duplicated alignments were found at 4087635 different positions |
Cytosine Methylation after Extraction
| Total C's analysed |
200,697,122 |
| Methylated C's in CpG context |
2,000,888 |
| Methylated C's in CHG context |
1,740,098 |
| Methylated C's in CHH context |
5,692,151 |
| Unmethylated C's in CpG context |
5,969,906 |
| Unmethylated C's in CHG context |
42,702,567 |
| Unmethylated C's in CHH context |
142,591,512 |
| Percentage methylation (CpG context) |
25.1% |
| Percentage methylation (CHG context) |
3.9% |
| Percentage methylation (CHH context) |
3.8% |
M-Bias Plot
