Alignment
| Sequences analysed in total |
40817621 |
| Single-end alignments with a unique best hit |
28343431 |
| Sequences without alignments under any condition |
4070690 |
| Sequences that did not map uniquely |
8403500 |
| Genomic sequence context not extractable (edges of chromosomes) |
6 |
Cytosine Methylation
| Total C's analysed |
252330135 |
| Methylated C's in CpG context |
3133999 |
| Methylated C's in CHG context |
2194953 |
| Methylated C's in CHH context |
7170938 |
| Methylated C's in Unknown context |
5 |
| Unmethylated C's in CpG context |
7824375 |
| Unmethylated C's in CHG context |
53581885 |
| Unmethylated C's in CHH context |
178423985 |
| Unmethylated C's in Unknown context |
37 |
| Percentage methylation (CpG context) |
28.6% |
| Percentage methylation (CHG context) |
3.9% |
| Percentage methylation (CHH context) |
3.9% |
| Methylated C's in Unknown context |
N/A% |
Alignment to Individual Bisulfite Strands
| OT |
14364802 |
original top strand |
| CTOT |
0 |
complementary to original top strand |
| CTOB |
0 |
complementary to original bottom strand |
| OB |
13978623 |
original bottom strand |
Deduplication
| Alignments analysed |
28343425 |
| Unique alignments |
22388069 |
| Duplicates removed |
5955356 |
| Duplicated alignments were found at 4087635 different positions |
Cytosine Methylation after Extraction
| Total C's analysed |
200697122 |
| Methylated C's in CpG context |
2000888 |
| Methylated C's in CHG context |
1740098 |
| Methylated C's in CHH context |
5692151 |
| Unmethylated C's in CpG context |
5969906 |
| Unmethylated C's in CHG context |
42702567 |
| Unmethylated C's in CHH context |
142591512 |
| Percentage methylation (CpG context) |
25.1% |
| Percentage methylation (CHG context) |
3.9% |
| Percentage methylation (CHH context) |
3.8% |
M-Bias Plot