Bismark

Bismark Processing Report

NF14_ES_+Ng_trimmed.fq

Data processed at 07:32 on 2016-11-30


Alignment

Sequences analysed in total 40817621
Single-end alignments with a unique best hit 28343431
Sequences without alignments under any condition 4070690
Sequences that did not map uniquely 8403500
Genomic sequence context not extractable (edges of chromosomes) 6

Cytosine Methylation

Total C's analysed 252330135
Methylated C's in CpG context 3133999
Methylated C's in CHG context 2194953
Methylated C's in CHH context 7170938
Methylated C's in Unknown context 5
Unmethylated C's in CpG context 7824375
Unmethylated C's in CHG context 53581885
Unmethylated C's in CHH context 178423985
Unmethylated C's in Unknown context 37
Percentage methylation (CpG context) 28.6%
Percentage methylation (CHG context) 3.9%
Percentage methylation (CHH context) 3.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 14364802 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 13978623 original bottom strand

Deduplication

Alignments analysed 28343425
Unique alignments 22388069
Duplicates removed 5955356
Duplicated alignments were found at 4087635 different positions

Cytosine Methylation after Extraction

Total C's analysed 200697122
Methylated C's in CpG context 2000888
Methylated C's in CHG context 1740098
Methylated C's in CHH context 5692151
Unmethylated C's in CpG context 5969906
Unmethylated C's in CHG context 42702567
Unmethylated C's in CHH context 142591512
Percentage methylation (CpG context) 25.1%
Percentage methylation (CHG context) 3.9%
Percentage methylation (CHH context) 3.8%

M-Bias Plot