Bismark

Bismark Processing Report

NF14_ES_N-_-_trimmed.fq

Data processed at 07:32 on 2016-11-30


Alignment

Sequences analysed in total 54945658
Single-end alignments with a unique best hit 38538424
Sequences without alignments under any condition 5363527
Sequences that did not map uniquely 11043707
Genomic sequence context not extractable (edges of chromosomes) 16

Cytosine Methylation

Total C's analysed 340799757
Methylated C's in CpG context 9131253
Methylated C's in CHG context 3280702
Methylated C's in CHH context 10311199
Methylated C's in Unknown context 1
Unmethylated C's in CpG context 5481679
Unmethylated C's in CHG context 71285272
Unmethylated C's in CHH context 241309652
Unmethylated C's in Unknown context 51
Percentage methylation (CpG context) 62.5%
Percentage methylation (CHG context) 4.4%
Percentage methylation (CHH context) 4.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 19531089 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 19007319 original bottom strand

Deduplication

Alignments analysed 38538408
Unique alignments 28958885
Duplicates removed 9579523
Duplicated alignments were found at 6513822 different positions

Cytosine Methylation after Extraction

Total C's analysed 257929130
Methylated C's in CpG context 6241643
Methylated C's in CHG context 2481088
Methylated C's in CHH context 7800367
Unmethylated C's in CpG context 3923096
Unmethylated C's in CHG context 54034280
Unmethylated C's in CHH context 183448656
Percentage methylation (CpG context) 61.4%
Percentage methylation (CHG context) 4.4%
Percentage methylation (CHH context) 4.1%

M-Bias Plot