Bismark

Bismark Processing Report

NF17_E14_EV_trimmed.fq

Data processed at 07:32 on 2016-11-30


Alignment

Sequences analysed in total 47321362
Single-end alignments with a unique best hit 33269648
Sequences without alignments under any condition 4983173
Sequences that did not map uniquely 9068541
Genomic sequence context not extractable (edges of chromosomes) 8

Cytosine Methylation

Total C's analysed 295249128
Methylated C's in CpG context 7123378
Methylated C's in CHG context 2769248
Methylated C's in CHH context 8779613
Methylated C's in Unknown context 6
Unmethylated C's in CpG context 5633520
Unmethylated C's in CHG context 62032993
Unmethylated C's in CHH context 208910376
Unmethylated C's in Unknown context 54
Percentage methylation (CpG context) 55.8%
Percentage methylation (CHG context) 4.3%
Percentage methylation (CHH context) 4.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 16853835 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 16415805 original bottom strand

Deduplication

Alignments analysed 33269640
Unique alignments 26191191
Duplicates removed 7078449
Duplicated alignments were found at 4865435 different positions

Cytosine Methylation after Extraction

Total C's analysed 234107426
Methylated C's in CpG context 5032491
Methylated C's in CHG context 2189132
Methylated C's in CHH context 6948454
Unmethylated C's in CpG context 4233865
Unmethylated C's in CHG context 49272988
Unmethylated C's in CHH context 166430496
Percentage methylation (CpG context) 54.3%
Percentage methylation (CHG context) 4.3%
Percentage methylation (CHH context) 4.0%

M-Bias Plot