Bismark

Bismark Processing Report

NF17_ES_+Ng_trimmed.fq

Data processed at 07:32 on 2016-11-30


Alignment

Sequences analysed in total 38145789
Single-end alignments with a unique best hit 26933611
Sequences without alignments under any condition 3844302
Sequences that did not map uniquely 7367876
Genomic sequence context not extractable (edges of chromosomes) 8

Cytosine Methylation

Total C's analysed 238792843
Methylated C's in CpG context 3870942
Methylated C's in CHG context 2074321
Methylated C's in CHH context 6853578
Methylated C's in Unknown context 6
Unmethylated C's in CpG context 6456949
Unmethylated C's in CHG context 50467641
Unmethylated C's in CHH context 169069412
Unmethylated C's in Unknown context 43
Percentage methylation (CpG context) 37.5%
Percentage methylation (CHG context) 3.9%
Percentage methylation (CHH context) 3.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 13644712 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 13288891 original bottom strand

Deduplication

Alignments analysed 26933603
Unique alignments 21633880
Duplicates removed 5299723
Duplicated alignments were found at 3657323 different positions

Cytosine Methylation after Extraction

Total C's analysed 193076545
Methylated C's in CpG context 2615440
Methylated C's in CHG context 1672010
Methylated C's in CHH context 5536277
Unmethylated C's in CpG context 5034591
Unmethylated C's in CHG context 40870492
Unmethylated C's in CHH context 137347735
Percentage methylation (CpG context) 34.2%
Percentage methylation (CHG context) 3.9%
Percentage methylation (CHH context) 3.9%

M-Bias Plot