Bismark

Bismark Processing Report

NF17_ES_+Ng_trimmed.fq

Data processed at 07:32 on 2016-11-30


Alignment

Sequences analysed in total 38,145,789
Single-end alignments with a unique best hit 26,933,611
Sequences without alignments under any condition 3,844,302
Sequences that did not map uniquely 7,367,876
Genomic sequence context not extractable (edges of chromosomes) 8
Created with Highcharts 3.0.4Unique Alignments: 70.6 %No Alignment: 10.1 %Multiple Alignments: 19.3 %No Genomic Sequence: 0.0 %

Cytosine Methylation

Total C's analysed 238,792,843
Methylated C's in CpG context 3,870,942
Methylated C's in CHG context 2,074,321
Methylated C's in CHH context 6,853,578
Methylated C's in Unknown context 6
Unmethylated C's in CpG context 6,456,949
Unmethylated C's in CHG context 50,467,641
Unmethylated C's in CHH context 169,069,412
Unmethylated C's in Unknown context 43
Percentage methylation (CpG context) 37.5%
Percentage methylation (CHG context) 3.9%
Percentage methylation (CHH context) 3.9%
Methylated C's in Unknown context N/A%
Created with Highcharts 3.0.4% MethylationCpGCHGCHH020406080100

Alignment to Individual Bisulfite Strands

OT 13,644,712 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 13,288,891 original bottom strand
Created with Highcharts 3.0.4Number of AlignmentsOTCTOTCTOBOB0M2.5M5M7.5M10M12.5M15M

Deduplication

Alignments analysed 26,933,603
Unique alignments 21,633,880
Duplicates removed 5,299,723
Duplicated alignments were found at 3657323 different positions
Created with Highcharts 3.0.4Unique: 80.3 %Duplicate: 19.7 %

Cytosine Methylation after Extraction

Total C's analysed 193,076,545
Methylated C's in CpG context 2,615,440
Methylated C's in CHG context 1,672,010
Methylated C's in CHH context 5,536,277
Unmethylated C's in CpG context 5,034,591
Unmethylated C's in CHG context 40,870,492
Unmethylated C's in CHH context 137,347,735
Percentage methylation (CpG context) 34.2%
Percentage methylation (CHG context) 3.9%
Percentage methylation (CHH context) 3.9%
Created with Highcharts 3.0.4% MethylationCpGCHGCHH020406080100

M-Bias Plot

Created with Highcharts 3.0.4Position (bp)% Methylation# Methylation CallsRead 1Click and drag in the plot area to zoom inCpG MethylationCHG MethylationCHH MethylationCpG Total CallsCHG Total CallsCHH Total Calls0510152025303540455001020304050607080901000k500k1,000k1,500k2,000k2,500k3,000k3,500k