Bismark

Bismark Processing Report

NF17_ES_N-_-_trimmed.fq

Data processed at 07:32 on 2016-11-30


Alignment

Sequences analysed in total 43875544
Single-end alignments with a unique best hit 31109899
Sequences without alignments under any condition 4378736
Sequences that did not map uniquely 8386909
Genomic sequence context not extractable (edges of chromosomes) 4

Cytosine Methylation

Total C's analysed 275566894
Methylated C's in CpG context 6742260
Methylated C's in CHG context 2533641
Methylated C's in CHH context 8095715
Methylated C's in Unknown context 4
Unmethylated C's in CpG context 5032926
Unmethylated C's in CHG context 57763701
Unmethylated C's in CHH context 195398651
Unmethylated C's in Unknown context 49
Percentage methylation (CpG context) 57.3%
Percentage methylation (CHG context) 4.2%
Percentage methylation (CHH context) 4.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15754450 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 15355445 original bottom strand

Deduplication

Alignments analysed 31109895
Unique alignments 24375160
Duplicates removed 6734735
Duplicated alignments were found at 4640423 different positions

Cytosine Methylation after Extraction

Total C's analysed 217388885
Methylated C's in CpG context 4766121
Methylated C's in CHG context 1998598
Methylated C's in CHH context 6394758
Unmethylated C's in CpG context 3770652
Unmethylated C's in CHG context 45618093
Unmethylated C's in CHH context 154840663
Percentage methylation (CpG context) 55.8%
Percentage methylation (CHG context) 4.2%
Percentage methylation (CHH context) 4.0%

M-Bias Plot