Bismark

Bismark Processing Report

NF17_Ng+Prd_trimmed.fq

Data processed at 07:32 on 2016-11-30


Alignment

Sequences analysed in total 51218055
Single-end alignments with a unique best hit 35971688
Sequences without alignments under any condition 5352935
Sequences that did not map uniquely 9893432
Genomic sequence context not extractable (edges of chromosomes) 17

Cytosine Methylation

Total C's analysed 318609382
Methylated C's in CpG context 1949810
Methylated C's in CHG context 2704803
Methylated C's in CHH context 8995531
Methylated C's in Unknown context 5
Unmethylated C's in CpG context 11666229
Unmethylated C's in CHG context 67300839
Unmethylated C's in CHH context 225992170
Unmethylated C's in Unknown context 42
Percentage methylation (CpG context) 14.3%
Percentage methylation (CHG context) 3.9%
Percentage methylation (CHH context) 3.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 18227489 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 17744182 original bottom strand

Deduplication

Alignments analysed 35971671
Unique alignments 28167955
Duplicates removed 7803716
Duplicated alignments were found at 5323605 different positions

Cytosine Methylation after Extraction

Total C's analysed 251284172
Methylated C's in CpG context 1117219
Methylated C's in CHG context 2130271
Methylated C's in CHH context 7103417
Unmethylated C's in CpG context 8704767
Unmethylated C's in CHG context 53161027
Unmethylated C's in CHH context 179067471
Percentage methylation (CpG context) 11.4%
Percentage methylation (CHG context) 3.9%
Percentage methylation (CHH context) 3.8%

M-Bias Plot