Bismark

Bismark Processing Report

NF17_Prd+EV_trimmed.fq

Data processed at 07:32 on 2016-11-30


Alignment

Sequences analysed in total 43766122
Single-end alignments with a unique best hit 30774537
Sequences without alignments under any condition 4408793
Sequences that did not map uniquely 8582792
Genomic sequence context not extractable (edges of chromosomes) 7

Cytosine Methylation

Total C's analysed 270463093
Methylated C's in CpG context 3738428
Methylated C's in CHG context 2384340
Methylated C's in CHH context 7948821
Methylated C's in Unknown context 6
Unmethylated C's in CpG context 7710169
Unmethylated C's in CHG context 56531256
Unmethylated C's in CHH context 192150079
Unmethylated C's in Unknown context 53
Percentage methylation (CpG context) 32.7%
Percentage methylation (CHG context) 4.0%
Percentage methylation (CHH context) 4.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15595058 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 15179472 original bottom strand

Deduplication

Alignments analysed 30774530
Unique alignments 23583932
Duplicates removed 7190598
Duplicated alignments were found at 4871300 different positions

Cytosine Methylation after Extraction

Total C's analysed 208742100
Methylated C's in CpG context 2433264
Methylated C's in CHG context 1834116
Methylated C's in CHH context 6132504
Unmethylated C's in CpG context 5606908
Unmethylated C's in CHG context 43707226
Unmethylated C's in CHH context 149028082
Percentage methylation (CpG context) 30.3%
Percentage methylation (CHG context) 4.0%
Percentage methylation (CHH context) 4.0%

M-Bias Plot