Bismark

Bismark Processing Report

NF17_ng+EV_trimmed.fq

Data processed at 07:32 on 2016-11-30


Alignment

Sequences analysed in total 41067195
Single-end alignments with a unique best hit 28225445
Sequences without alignments under any condition 4889856
Sequences that did not map uniquely 7951894
Genomic sequence context not extractable (edges of chromosomes) 9

Cytosine Methylation

Total C's analysed 248788324
Methylated C's in CpG context 2734772
Methylated C's in CHG context 2164429
Methylated C's in CHH context 7271896
Methylated C's in Unknown context 4
Unmethylated C's in CpG context 7930762
Unmethylated C's in CHG context 52183606
Unmethylated C's in CHH context 176502859
Unmethylated C's in Unknown context 47
Percentage methylation (CpG context) 25.6%
Percentage methylation (CHG context) 4.0%
Percentage methylation (CHH context) 4.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 14326472 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 13898964 original bottom strand

Deduplication

Alignments analysed 28225436
Unique alignments 21580762
Duplicates removed 6644674
Duplicated alignments were found at 4431191 different positions

Cytosine Methylation after Extraction

Total C's analysed 191753930
Methylated C's in CpG context 1619800
Methylated C's in CHG context 1661368
Methylated C's in CHH context 5604286
Unmethylated C's in CpG context 5820743
Unmethylated C's in CHG context 40271429
Unmethylated C's in CHH context 136776304
Percentage methylation (CpG context) 21.8%
Percentage methylation (CHG context) 4.0%
Percentage methylation (CHH context) 3.9%

M-Bias Plot