Bismark

Bismark Processing Report

Nf14_trimmed.fq

Data processed at 07:32 on 2016-11-30


Alignment

Sequences analysed in total 38694728
Single-end alignments with a unique best hit 26775823
Sequences without alignments under any condition 4522753
Sequences that did not map uniquely 7396152
Genomic sequence context not extractable (edges of chromosomes) 7

Cytosine Methylation

Total C's analysed 236830909
Methylated C's in CpG context 1521136
Methylated C's in CHG context 2019905
Methylated C's in CHH context 6805531
Methylated C's in Unknown context 3
Unmethylated C's in CpG context 8435386
Unmethylated C's in CHG context 49508859
Unmethylated C's in CHH context 168540092
Unmethylated C's in Unknown context 36
Percentage methylation (CpG context) 15.3%
Percentage methylation (CHG context) 3.9%
Percentage methylation (CHH context) 3.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 13563366 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 13212450 original bottom strand

Deduplication

Alignments analysed 26775816
Unique alignments 20306675
Duplicates removed 6469141
Duplicated alignments were found at 4389179 different positions

Cytosine Methylation after Extraction

Total C's analysed 180906565
Methylated C's in CpG context 812836
Methylated C's in CHG context 1541274
Methylated C's in CHH context 5205510
Unmethylated C's in CpG context 6123570
Unmethylated C's in CHG context 37882671
Unmethylated C's in CHH context 129340704
Percentage methylation (CpG context) 11.7%
Percentage methylation (CHG context) 3.9%
Percentage methylation (CHH context) 3.9%

M-Bias Plot