Bismark

Bismark Processing Report

NfV_+2_trimmed.fq

Data processed at 07:32 on 2016-11-30


Alignment

Sequences analysed in total 42580713
Single-end alignments with a unique best hit 29298801
Sequences without alignments under any condition 4427565
Sequences that did not map uniquely 8854347
Genomic sequence context not extractable (edges of chromosomes) 8

Cytosine Methylation

Total C's analysed 258783988
Methylated C's in CpG context 2486910
Methylated C's in CHG context 2227062
Methylated C's in CHH context 7545918
Methylated C's in Unknown context 2
Unmethylated C's in CpG context 8412744
Unmethylated C's in CHG context 53845778
Unmethylated C's in CHH context 184265576
Unmethylated C's in Unknown context 44
Percentage methylation (CpG context) 22.8%
Percentage methylation (CHG context) 4.0%
Percentage methylation (CHH context) 3.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 14862071 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 14436722 original bottom strand

Deduplication

Alignments analysed 29298793
Unique alignments 21661854
Duplicates removed 7636939
Duplicated alignments were found at 5065741 different positions

Cytosine Methylation after Extraction

Total C's analysed 192733908
Methylated C's in CpG context 1381716
Methylated C's in CHG context 1655628
Methylated C's in CHH context 5621688
Unmethylated C's in CpG context 5960458
Unmethylated C's in CHG context 40175798
Unmethylated C's in CHH context 137938620
Percentage methylation (CpG context) 18.8%
Percentage methylation (CHG context) 4.0%
Percentage methylation (CHH context) 3.9%

M-Bias Plot