Bismark

Bismark Processing Report

NfV_+3_trimmed.fq

Data processed at 07:32 on 2016-11-30


Alignment

Sequences analysed in total 37295107
Single-end alignments with a unique best hit 25679235
Sequences without alignments under any condition 4374229
Sequences that did not map uniquely 7241643
Genomic sequence context not extractable (edges of chromosomes) 3

Cytosine Methylation

Total C's analysed 226950434
Methylated C's in CpG context 3317024
Methylated C's in CHG context 1979125
Methylated C's in CHH context 6574055
Methylated C's in Unknown context 5
Unmethylated C's in CpG context 6495487
Unmethylated C's in CHG context 47921019
Unmethylated C's in CHH context 160663724
Unmethylated C's in Unknown context 44
Percentage methylation (CpG context) 33.8%
Percentage methylation (CHG context) 4.0%
Percentage methylation (CHH context) 3.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 13043896 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 12635336 original bottom strand

Deduplication

Alignments analysed 25679232
Unique alignments 20008408
Duplicates removed 5670824
Duplicated alignments were found at 3847579 different positions

Cytosine Methylation after Extraction

Total C's analysed 178130878
Methylated C's in CpG context 2029861
Methylated C's in CHG context 1545657
Methylated C's in CHH context 5152668
Unmethylated C's in CpG context 4947513
Unmethylated C's in CHG context 37672021
Unmethylated C's in CHH context 126783158
Percentage methylation (CpG context) 29.1%
Percentage methylation (CHG context) 3.9%
Percentage methylation (CHH context) 3.9%

M-Bias Plot