FastQCFastQC Report
Fri 29 Apr 2016
E14_K27.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameE14_K27.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28129717
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT16800.021.35109742
TCTCGTA18700.019.65325741
TCGTATG19350.018.6510843
GATCGGA21950.018.3526941
CGCCAAT21400.018.3029533
CGTATGC20150.018.23809244
ACGCCAA22150.018.08032832
TATCTCG21750.017.30228639
GTCACGC23450.016.79402429
ATCGGAA24200.016.726572
ATCTCGT24900.015.11322740
CGTCTGA27400.014.52926416
TCACGCC29250.013.61436130
ACACGTC30400.013.31253213
AGTCACG30850.012.83683828
ACGTCTG31950.012.73551515
CACGCCA31800.012.66202931
TCGGAAG35450.011.72870353
CGGAAGA38400.011.0568284
CACGTCT40800.010.026957514
Produced by FastQC (version 0.11.3)