FastQCFastQC Report
Fri 29 Apr 2016
E14_inp.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameE14_inp.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46259566
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA42250.031.626011
TCTCGTA42100.029.79727441
CGATGTA42550.029.48013734
CGTATGC43550.028.4997144
ATCGGAA47100.028.3973732
ACCGATG45200.028.1867232
CACCGAT45150.028.1672531
CTCGTAT44100.028.04501542
TCGTATG44600.027.92758843
TATCTCG46750.026.8811439
CCGATGT50100.025.43040333
ACACGTC54250.024.28502513
CGTCTGA53800.024.24283416
GTCACCG54400.023.6591429
ATCTCGT54600.022.93529740
ACGTCTG58500.022.558315
TCGGAAG59600.022.1462653
TCACCGA61500.020.96353330
CGGAAGA66250.019.9564884
CACGTCT73950.018.0237814
Produced by FastQC (version 0.11.3)