FastQCFastQC Report
Fri 29 Apr 2016
EVx2_K27.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameEVx2_K27.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33028918
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT19550.021.72533242
TCTCGTA20950.020.37992341
TCGTATG20900.020.2164543
CGTATGC21300.020.04331244
GATCGGA25350.018.0599941
ATCGGAA27550.016.7680022
CGTCTGA30800.014.5676716
ATCTCGT30200.014.50214640
ACACGTC32050.014.27402913
ACGTCTG34950.013.34133415
CATCTCG33300.012.95409939
TCGGAAG37900.012.1888753
CACGTCT46150.010.38953314
CGGAAGA47050.09.8652124
CACACGT54250.08.91934412
GCACACG60050.08.13111111
GATCATC62800.07.709966736
ACCAGAT94150.05.819525232
AGATCAT89900.05.777480635
AGTCACC98050.05.34029128
Produced by FastQC (version 0.11.3)