FastQCFastQC Report
Fri 29 Apr 2016
EVx2_inp.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameEVx2_inp.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46198038
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA37350.029.586071
TCTCGTA36000.027.20412341
CTCGTAT37300.026.3135442
ATCGGAA43300.025.6056882
TCGTATG39100.025.32700343
CGTATGC40050.024.89094444
AATCTCG41100.024.15002439
CGTCTGA45250.024.01187516
ACACGTC49400.022.17277713
ACGTCTG53650.020.29332415
ATCTCGT50450.020.02300540
TCGGAAG55750.019.8874683
CGGAAGA60000.018.4421084
CACGTCT67550.016.24773814
GCACACG78350.014.26068911
CACACGT83050.013.55957612
ACCAATC112600.09.49880536
GACCAAT119250.09.15359235
ACTGACC130400.08.58894432
CCAATCT131850.08.22882637
Produced by FastQC (version 0.11.3)