FastQCFastQC Report
Fri 29 Apr 2016
Nx2_K27.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameNx2_K27.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33203021
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT20900.022.74290742
GATCGGA23000.022.3002241
TCTCGTA21300.022.0072641
TCGTATG21300.022.0055743
ATCGGAA26000.019.9683592
CGTATGC23850.019.9294244
AATCTCG28550.016.80434439
ATCTCGT30000.016.28537240
ACACGTC31800.016.25384513
CGTCTGA31550.015.75519816
ACGTCTG35450.014.39416615
TCGGAAG40200.013.5168233
CACGTCT43700.012.02906614
CGGAAGA46800.011.657614
CACACGT56050.09.57479812
GCACACG62250.08.55049811
GTAATCT72450.07.381327637
TGTAATC89550.06.04555436
CCTTGTA96350.05.93784333
GAACTCC103850.05.82456121
Produced by FastQC (version 0.11.3)