FastQCFastQC Report
Fri 29 Apr 2016
Nx2_inp.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameNx2_inp.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36320699
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA35500.034.848971
ATCGGAA40050.030.7050722
TCGTATG39400.029.2669243
TCTCGTA39450.029.17516941
CTCGTAT39750.029.00942442
CGTATGC40250.028.7581744
GATCTCG42400.027.6648239
CGTCTGA43650.027.66360916
ACACGTC45600.027.1559413
ACGTCTG47750.025.65680115
TCGGAAG49700.024.6989573
ATCTCGT50000.023.67941740
CGGAAGA52650.023.48224
CACGTCT61150.020.46619614
GCACACG68550.018.35309411
CACACGT70150.018.0912612
AGTGATC98250.012.386559536
GTGATCT108050.011.20201337
GAACTCC118200.010.71870821
GTCACAC118250.010.41905629
Produced by FastQC (version 0.11.3)