FastQCFastQC Report
Fri 29 Apr 2016
Histon_modification_E14.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHiston_modification_E14.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75608705
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT3435520.45438154244276496No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG2983810.39463842159444473No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA2856800.3778400912963659No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT2346350.31032802373747836No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA2302480.30452578178663425No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA1935630.256006236318953No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC1932170.2555486170540813No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT1594200.210848737589144No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT1537230.20331389090713828No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT1491810.19730664610642387No Hit
CGGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTA1399350.1850778954619048No Hit
GTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTA1366640.18075167403012654No Hit
CTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAA1338790.17706823572761893No Hit
CTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCATTC1250940.165449203236585No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA1142470.15110297154276087No Hit
CGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATATATCTGGGT1057540.13987013796890185No Hit
CGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTA1035950.13701464666006383No Hit
GCTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTT1021170.13505984529162351No Hit
CAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAATCA1005950.13304684956580065No Hit
GTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTC961000.12710176691956301No Hit
CTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAAGG932360.12331384329357314No Hit
CTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGACAGTTGGACC928220.12276628729456483No Hit
ATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCTCCATAG899270.11893736309860087No Hit
CCGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTG888190.11747192337178634No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT888040.11745208438631505No Hit
GTGTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGC870280.11510314850651125No Hit
GTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAATACTTGTAATGCTA844300.11166703622287935No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT837640.11078618526795293No Hit
CGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCA823070.10885915847917246No Hit
CTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGTAAATAGATAGAA811800.10736858937076095No Hit
CTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGC802400.10612534628122515No Hit
GTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAAGGTA793360.10492971675682053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA472250.049.818321
TACGTAT502750.047.019672
TCGTTTG1025250.044.519972
ACGTATA535500.044.033083
CGTTTGG1108950.039.487853
GTGTACG285300.038.2201651
GTTTCGG1174500.037.7595949
CGTATAT666800.036.4497574
GGTTTCG1234800.035.9348958
GTTCGTT124900.033.797991
CTCGTTT1748050.033.5603331
TGTACGT327700.033.239382
GGAGTCT1151950.033.123483
CGTTGAT1082800.032.918191
GAGTCTT1206250.031.844244
CGCCGTT83100.031.552411
AGTCTTG1323550.029.9379565
TGGAGTC1295700.029.3935952
CTCGTGT265750.028.749141
CCCGTTT291750.028.5103031
Produced by FastQC (version 0.11.3)