FastQCFastQC Report
Fri 29 Apr 2016
Histon_modification_E14.r_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHiston_modification_E14.r_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75608705
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAATATAATTATACTATTATATAAATCAAAACATTTATCCTACTAAA5545410.7334353894832613No Hit
CTTAAATTATATAACTTATCTATTTAATTTATTAAACCTAATGGCCCAAA1796130.2375559798306293No Hit
CCTACACAAATATAATTATACTATTATATAAATCAAAACATTTATCCTAC1755660.23220342155046828No Hit
CTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAGC1715410.22687996044899855No Hit
CTGAAATATAATAATAAGACGAGAAGACCCTATGGAGCTTAAATTATATA1643740.21740089319080388No Hit
CCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAAACATTTA1384240.1830794483254276No Hit
CGACAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGT1187590.15707053837253265No Hit
CTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTC991000.1310695640138262No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT936590.12387330268386425No Hit
CAAAAAATGAATTTAAGTTCAATTTTAAACTTGCTAAAAAAACAACAAAA886730.11727882391319888No Hit
CCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTT870040.11507140612975715No Hit
CCTCGATGTTGGATCAGGACATCCCAATGGTGTAGAAGCTATTAATGGTT864470.11433471846925562No Hit
CTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATG844540.11169877859963347No Hit
CTGTCTCTTATCTTTAATCAGTGAAATTGACCTTTCAGTGAAGAGGCTGA831680.10999791624522601No Hit
CTATTATATAAATCAAAACATTTATCCTACTAAAAGTATTGGAGAAAGAA817410.10811056742738814No Hit
CCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTT799220.10570475978923326No Hit
GGAAGCTCATCAGCGGGGCCACGTGCTGAGTGCTCGTCACTCTTCGGCCC777900.10288497865424359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAAAT1577700.045.7527771
CAAATAT1697750.041.543923
ACAAATA1779600.039.2135352
GCGCACG301350.035.1224376
CTAGCCC494000.034.9428522
TAATTAT1999550.034.5196159
GCGACAT275050.033.1858187
AATATAA2787000.032.7780345
TATAATT2162150.032.6405377
ATATAAT2796900.032.322236
AAATATA2878750.031.987124
TGCGACA285350.031.6144666
GGCGCAC341150.031.0911525
CGCACGC397950.030.9165481
TAGCCCT564300.030.6307983
CGGTGGC556950.027.7597871
CCTAGCC653050.027.344371
CCTACAC915350.026.6611671
ATCAGCG374250.026.3315549
GCCCTAC659650.026.2371085
Produced by FastQC (version 0.11.3)