FastQCFastQC Report
Fri 29 Apr 2016
Histon_modification_EVx2.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHiston_modification_EVx2.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71293524
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA2578720.3617046619830435No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT2540170.3562974387407193No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG2253800.31612969503373123No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT2090490.2932229861438747No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA2015440.2826960833076508No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC1699260.23834703415698738No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT1566180.21968054209243465No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA1559400.2187295440747185No Hit
CGGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTA1337290.18757524175688103No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT1333660.18706607910137812No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT1206930.1692902710209696No Hit
GTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTA1108900.15554007401850412No Hit
CTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAA1060350.14873019883264574No Hit
CTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCATTC1009110.14154301027397664No Hit
CGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATATATCTGGGT940740.1319530789360335No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA927410.13008334389530246No Hit
CGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTA893900.12538305723251947No Hit
GCTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTT860580.12070942095666361No Hit
GTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAATACTTGTAATGCTA840430.11788307729044226No Hit
GTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTC837540.11747771087876088No Hit
CAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAATCA829290.1163205230253452No Hit
CCGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTG814730.11427826179555944No Hit
CTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGACAGTTGGACC789570.11074918950562747No Hit
ATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCTCCATAG761420.10680072428457876No Hit
CTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGC756630.1061288540036259No Hit
GTGTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGC756470.10610641157252937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA424650.049.9573021
TACGTAT450850.047.3852922
ACGTATA479900.044.44263
TCGTTTG870100.038.4927142
GTGTACG253750.037.3263051
CGTATAT590450.037.279034
CGTTTGG941400.034.4410933
GTTTCGG977900.033.574899
CGTTGAT975900.032.9485741
TGTACGT293950.032.386632
GGTTTCG1026650.031.992188
CGCCGTT77300.031.4593031
GGAGTCT989250.029.723533
CTCGTTT1536450.029.5728931
GAGTCTT1021750.029.0630664
CCCGATT150950.028.4386731
CCCGTTT277600.028.100861
GTTCGTT119850.027.632641
TGATCAA1263750.027.353614
AGTCTTG1132050.026.9925065
Produced by FastQC (version 0.11.3)