FastQCFastQC Report
Fri 29 Apr 2016
Histon_modification_EVx2.r_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHiston_modification_EVx2.r_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71293524
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAATATAATTATACTATTATATAAATCAAAACATTTATCCTACTAAA4360900.6116824860558162No Hit
CTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAGC1532510.2149578129985551No Hit
CCTACACAAATATAATTATACTATTATATAAATCAAAACATTTATCCTAC1488020.2087174145017716No Hit
CTGAAATATAATAATAAGACGAGAAGACCCTATGGAGCTTAAATTATATA1403230.19682432867254535No Hit
CTTAAATTATATAACTTATCTATTTAATTTATTAAACCTAATGGCCCAAA1316820.1847040132284666No Hit
CCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAAACATTTA1219930.17111371854756402No Hit
CGACAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGT1015490.14243790221395145No Hit
CTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTC862200.12093665057151615No Hit
CAAAAAATGAATTTAAGTTCAATTTTAAACTTGCTAAAAAAACAACAAAA809730.11357693582379234No Hit
CCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTT751230.10537142195411746No Hit
CTGTCTCTTATCTTTAATCAGTGAAATTGACCTTTCAGTGAAGAGGCTGA749500.10512876316788604No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT720480.1010582672277499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAAAT1384050.041.44651
CAAATAT1470550.037.982723
ACAAATA1568600.035.2721942
CTAGCCC470550.033.957592
GCGCACG287200.032.9435166
GCGACAT240950.031.1999657
TAATTAT1767350.030.8368199
TATAATT1893000.029.572697
AATATAA2471500.029.5677935
TAGCCCT543950.029.4291423
ATATAAT2480850.029.1110946
TGCGACA254300.028.8857336
AAATATA2576000.028.5477244
GGCGCAC332100.028.5037385
CGGTGGC524000.027.1016271
CGCACGC358900.027.0254461
GCCCTAC594250.026.9160125
CCTAGCC652200.025.5766931
AGCCCTA640200.025.2921054
ATCAGCG335100.025.0810179
Produced by FastQC (version 0.11.3)