FastQCFastQC Report
Fri 29 Apr 2016
Histon_modification_Nx2.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHiston_modification_Nx2.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70139354
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG2556280.3644573059512353No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1225740.17475781142780414No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1092960.15582692706294388No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT766730.1093152354953255No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG711840.10148938640067887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA149750.036.5282361
TACGTAT155700.035.074682
ACGTATA160900.033.6456683
GGAGTCT1091500.030.3574893
GAGTCTT1126800.029.6279584
CCCGTTT278550.029.1364881
CTCGCTA142550.029.11361
CGCGTAA12450.028.654291
CGTATAT194250.028.6033824
TGGAGTC1200350.027.6244182
AGTCTTG1276250.027.159585
CTCGTTT563350.027.1562161
CCCGTAT109750.026.9794161
TCGCTAT149100.026.8228052
TCGTTTG360850.026.7275162
CCCGAAT184500.026.2322641
CCTACGT666100.025.66657824-25
TACGTTC671800.025.48628226-27
GTCTTGG1406100.025.0360416
CTACGTT675000.024.9911426-27
Produced by FastQC (version 0.11.3)