FastQCFastQC Report
Fri 29 Apr 2016
Histon_modification_Nx2.r_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHiston_modification_Nx2.r_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70139354
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT1662610.23704381423301962No Hit
CCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTT1404090.20018576161964652No Hit
CACAAATATAATTATACTATTATATAAATCAAAACATTTATCCTACTAAA932680.1329752766186013No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG891210.12706276137074202No Hit
GGAAGCTCATCAGCGGGGCCACGTGCTGAGTGCTCGTCACTCTTCGGCCC879300.12536471322504625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC706500.039.871971
GCGCACG472800.038.387936
GGTGGCG792200.034.2512132
GGCGCAC530600.034.222685
CGCACGC644600.033.783491
TGGCGCA575850.031.6489814
GTGGCGC649250.030.9474223
TGCGACA272050.030.932696
GCGACAT276150.030.8595967
CGGTGGT518450.030.2697581
ATCAGCG422550.027.6550439
GCGGACG199800.027.4839826
GCACGCC830400.025.9585842
CACGCCT843250.025.5481933
CACAAAT645400.022.6664031
TGTAGTC937550.022.2589829
ACGCCTG965150.021.8526944
GGCGATC118850.021.5644512
GACGTCT155700.021.4925869
CCTGTAG989200.021.272967
Produced by FastQC (version 0.11.3)