Bismark

Bismark Processing Report

cp_SLX-10773.RPIBS49.C80Y3ANXX.s_5.r_1_trimmed.fq

Data processed at 16:01 on 2016-01-22


Alignment

Sequences analysed in total 41293576
Single-end alignments with a unique best hit 29491281
Sequences without alignments under any condition 3255325
Sequences that did not map uniquely 8546970
Genomic sequence context not extractable (edges of chromosomes) 7

Cytosine Methylation

Total C's analysed 267197444
Methylated C's in CpG context 5762426
Methylated C's in CHG context 374598
Methylated C's in CHH context 888894
Methylated C's in Unknown context 3
Unmethylated C's in CpG context 5506907
Unmethylated C's in CHG context 57729663
Unmethylated C's in CHH context 196934956
Unmethylated C's in Unknown context 66
Percentage methylation (CpG context) 51.1%
Percentage methylation (CHG context) 0.6%
Percentage methylation (CHH context) 0.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 14965353 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 14525921 original bottom strand

Deduplication

Alignments analysed 29491274
Unique alignments 27533935
Duplicates removed 1957339
Duplicated alignments were found at 873673 different positions

Cytosine Methylation after Extraction

Total C's analysed 251238475
Methylated C's in CpG context 4756156
Methylated C's in CHG context 350283
Methylated C's in CHH context 823149
Unmethylated C's in CpG context 4863676
Unmethylated C's in CHG context 54258630
Unmethylated C's in CHH context 186186581
Percentage methylation (CpG context) 49.4%
Percentage methylation (CHG context) 0.6%
Percentage methylation (CHH context) 0.4%

M-Bias Plot