Bismark

Bismark Processing Report

cp_SLX-10773.RPI44.C80Y3ANXX.s_5.r_1_trimmed.fq

Data processed at 16:01 on 2016-01-22


Alignment

Sequences analysed in total 42062207
Single-end alignments with a unique best hit 30164086
Sequences without alignments under any condition 3447260
Sequences that did not map uniquely 8450861
Genomic sequence context not extractable (edges of chromosomes) 11

Cytosine Methylation

Total C's analysed 272510191
Methylated C's in CpG context 6058625
Methylated C's in CHG context 378396
Methylated C's in CHH context 883462
Methylated C's in Unknown context 5
Unmethylated C's in CpG context 5533683
Unmethylated C's in CHG context 59077001
Unmethylated C's in CHH context 200579024
Unmethylated C's in Unknown context 33
Percentage methylation (CpG context) 52.3%
Percentage methylation (CHG context) 0.6%
Percentage methylation (CHH context) 0.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15316287 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 14847788 original bottom strand

Deduplication

Alignments analysed 30164075
Unique alignments 28103809
Duplicates removed 2060266
Duplicated alignments were found at 887454 different positions

Cytosine Methylation after Extraction

Total C's analysed 255792659
Methylated C's in CpG context 4991396
Methylated C's in CHG context 352303
Methylated C's in CHH context 814044
Unmethylated C's in CpG context 4837283
Unmethylated C's in CHG context 55438894
Unmethylated C's in CHH context 189358739
Percentage methylation (CpG context) 50.8%
Percentage methylation (CHG context) 0.6%
Percentage methylation (CHH context) 0.4%

M-Bias Plot