Bismark

Bismark Processing Report

cp_SLX-10773.RPIBS41.C80Y3ANXX.s_5.r_1_trimmed.fq

Data processed at 16:01 on 2016-01-22


Alignment

Sequences analysed in total 41838389
Single-end alignments with a unique best hit 30254168
Sequences without alignments under any condition 3263888
Sequences that did not map uniquely 8320333
Genomic sequence context not extractable (edges of chromosomes) 6

Cytosine Methylation

Total C's analysed 273912876
Methylated C's in CpG context 2646309
Methylated C's in CHG context 188393
Methylated C's in CHH context 546529
Methylated C's in Unknown context 2
Unmethylated C's in CpG context 8828062
Unmethylated C's in CHG context 59384428
Unmethylated C's in CHH context 202319155
Unmethylated C's in Unknown context 54
Percentage methylation (CpG context) 23.1%
Percentage methylation (CHG context) 0.3%
Percentage methylation (CHH context) 0.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15335181 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 14918981 original bottom strand

Deduplication

Alignments analysed 30254162
Unique alignments 28012477
Duplicates removed 2241685
Duplicated alignments were found at 1159965 different positions

Cytosine Methylation after Extraction

Total C's analysed 255399048
Methylated C's in CpG context 1870614
Methylated C's in CHG context 172704
Methylated C's in CHH context 501495
Unmethylated C's in CpG context 7887387
Unmethylated C's in CHG context 55352879
Unmethylated C's in CHH context 189613969
Percentage methylation (CpG context) 19.2%
Percentage methylation (CHG context) 0.3%
Percentage methylation (CHH context) 0.3%

M-Bias Plot