Bismark

Bismark Processing Report

cp_SLX-10773.RPI31.C80Y3ANXX.s_5.r_1_trimmed.fq

Data processed at 16:01 on 2016-01-22


Alignment

Sequences analysed in total 37721665
Single-end alignments with a unique best hit 27212362
Sequences without alignments under any condition 3072169
Sequences that did not map uniquely 7437134
Genomic sequence context not extractable (edges of chromosomes) 9

Cytosine Methylation

Total C's analysed 247843938
Methylated C's in CpG context 1269468
Methylated C's in CHG context 169864
Methylated C's in CHH context 496313
Methylated C's in Unknown context 2
Unmethylated C's in CpG context 9257661
Unmethylated C's in CHG context 53972426
Unmethylated C's in CHH context 182678206
Unmethylated C's in Unknown context 51
Percentage methylation (CpG context) 12.1%
Percentage methylation (CHG context) 0.3%
Percentage methylation (CHH context) 0.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 13811121 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 13401232 original bottom strand

Deduplication

Alignments analysed 27212353
Unique alignments 25416352
Duplicates removed 1796001
Duplicated alignments were found at 785583 different positions

Cytosine Methylation after Extraction

Total C's analysed 233196766
Methylated C's in CpG context 760133
Methylated C's in CHG context 157183
Methylated C's in CHH context 460054
Unmethylated C's in CpG context 8236045
Unmethylated C's in CHG context 50773221
Unmethylated C's in CHH context 172810130
Percentage methylation (CpG context) 8.4%
Percentage methylation (CHG context) 0.3%
Percentage methylation (CHH context) 0.3%

M-Bias Plot