FastQCFastQC Report
Tue 8 Dec 2015
SLX-10776.A002.C80FNANXX.s_8.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10776.A002.C80FNANXX.s_8.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30256808
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG969110.32029485727641854No Hit
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT948430.3134600318711742No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA927980.30670122241579484No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA870930.2878459618080004No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT718870.237589503823404No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA632020.20888522014615687No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT621650.20545789231963926No Hit
CTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAA553880.18305962743988063No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC546690.1806833027462778No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA530580.1753588812144361No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT502070.1659362084724866No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT477730.15789173795200073No Hit
CTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAAGG431790.14270837822681096No Hit
GTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTC424930.1404411198960578No Hit
CTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGTAAATAGATAGAA391020.12923372485293227No Hit
CAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAATCA369230.12203203986355732No Hit
ATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCTCCATAG364360.12042248475120046No Hit
CGGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTA336570.11123777498274108No Hit
CTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGC332190.10979016689400944No Hit
CCGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTG329800.10900026202367413No Hit
CGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTA319590.10562581485793214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA170950.040.0172121
TCGTTTG334500.038.7817462
TACGTAT181000.038.1195072
ACGTATA192900.035.70663
GTTTCGG356950.034.9256559
CGTTTGG354550.034.861033
GGTTTCG380950.032.69418
CGTATAT234350.030.2537484
GTGTACG117500.030.0216751
CGTTGAT365300.029.6536661
CTCGTTT627150.029.6412621
CTCGTGT109700.029.3365271
GGAGTCT426300.028.6587523
GAGTCTT432400.028.611824
CTCCGTT253600.027.7728581
CTCGCTA34550.027.0314711
AGTCTTG472800.026.8714835
TGTACGT136350.025.999142
CCTACGT242950.025.96464724-25
TGGAGTC471600.025.842592
Produced by FastQC (version 0.11.2)