FastQCFastQC Report
Tue 8 Dec 2015
SLX-10776.A002.C80FNANXX.s_8.r_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10776.A002.C80FNANXX.s_8.r_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30256808
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAATATAATTATACTATTATATAAATCAAAACATTTATCCTACTAAA1806470.5970457954454416No Hit
CTTAAATTATATAACTTATCTATTTAATTTATTAAACCTAATGGCCCAAA691010.22838165876585526No Hit
CTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAGC605810.20022270690285637No Hit
CCTACACAAATATAATTATACTATTATATAAATCAAAACATTTATCCTAC477020.1576570800198091No Hit
CTGAAATATAATAATAAGACGAGAAGACCCTATGGAGCTTAAATTATATA474950.15697293647102495No Hit
CCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAAACATTTA448370.14818813669968095No Hit
CGACAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGT384700.12714493875229665No Hit
CTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTC343810.11363062488283628No Hit
CAAAAAATGAATTTAAGTTCAATTTTAAACTTGCTAAAAAAACAACAAAA339000.11204090001827027No Hit
CCTCGATGTTGGATCAGGACATCCCAATGGTGTAGAAGCTATTAATGGTT307650.10167959554755411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAAAT546050.042.2324871
CAAATAT592900.037.7210853
GCGCACG109600.034.3708086
ACAAATA644350.034.2753262
TAATTAT704300.031.183759
GGCGCAC121850.030.8726835
GCGACAT106650.029.9071757
AATATAA942550.029.8071465
ATATAAT945150.029.4988776
TATAATT774300.028.9352447
CGGTGGC224250.028.8546941
AAATATA983700.028.7441624
TGCGACA116300.027.2737036
CTAGCCC194550.026.9265612
GTGGCGC183700.026.7991373
GGCGATC42900.026.0873552
CGCACGC135900.025.703377
TGGCGCA153050.024.8534244
GGTGGCG239650.023.8712642
TAGCCCT222300.023.4578973
Produced by FastQC (version 0.11.2)