FastQCFastQC Report
Tue 8 Dec 2015
SLX-10776.A004.C80FNANXX.s_8.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10776.A004.C80FNANXX.s_8.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30747616
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT909980.2959513999394294No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA843040.2741806063923785No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA810390.26356189696137744No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG766000.24912500533374685No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT664290.2160460180067294No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA539850.17557458763632275No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT539110.17533391857111785No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA525140.17079047689420865No Hit
CTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAA470120.15289640666775595No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC456510.14847004723878432No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT445410.1448600112607104No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT445080.14475268586676768No Hit
GTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTC424100.13792939263974155No Hit
CTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAAGG402850.1310182877267623No Hit
CGGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTA345510.11236968745804554No Hit
CAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAATCA334790.10888323829723905No Hit
ATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCTCCATAG320790.10433003976633506No Hit
CTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGTAAATAGATAGAA320370.10419344381040793No Hit
CCGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTG309320.1005996692556587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA150350.040.1192631
TCGTTTG323100.038.9906122
TACGTAT159600.038.125352
ACGTATA171350.035.3033333
GTTTCGG340100.034.8202749
CGTTTGG350750.033.9843443
GGTTTCG365000.032.5100488
CTCGTTT584750.030.1425381
CGTATAT214950.029.3874364
CGTTGAT343100.029.3704811
CTCCGTT243950.028.4081881
CCCGATT54400.028.3216951
CTCGTGT107100.028.271361
GTGTACG108450.027.9194361
CTCGCTA33500.027.8787481
GGAGTCT390900.026.2483653
CCCGTAT44700.026.0835651
TACGTTC201950.025.94865226-27
CCTACGT202100.025.89989924-25
GAGTCTT399700.025.8636744
Produced by FastQC (version 0.11.2)