FastQCFastQC Report
Tue 8 Dec 2015
SLX-10776.A004.C80FNANXX.s_8.r_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10776.A004.C80FNANXX.s_8.r_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30747616
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAATATAATTATACTATTATATAAATCAAAACATTTATCCTACTAAA1685540.5481855894128507No Hit
CTTAAATTATATAACTTATCTATTTAATTTATTAAACCTAATGGCCCAAA642530.2089690465758386No Hit
CTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAAAACAGC581440.18910083955777254No Hit
CCTACACAAATATAATTATACTATTATATAAATCAAAACATTTATCCTAC453780.14758217352525801No Hit
CTGAAATATAATAATAAGACGAGAAGACCCTATGGAGCTTAAATTATATA439930.143077759264328No Hit
CCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAAACATTTA396660.12900512351916973No Hit
CGACAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGT338020.1099337262440119No Hit
CTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTC316640.10298034163038852No Hit
CAAAAAATGAATTTAAGTTCAATTTTAAACTTGCTAAAAAAACAACAAAA315160.10249900349997866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAAAT534700.041.9728361
CAAATAT571900.037.6713033
ACAAATA621900.034.317392
TAATTAT654550.032.3189
GCGCACG95550.032.2628976
AATATAA891900.030.3394745
ATATAAT890000.029.8897176
TATAATT724550.029.5672447
AAATATA927250.029.0509974
GGCGCAC109000.028.4515515
GCGACAT90350.027.333597
CTAGCCC179300.026.4952622
CGGTGGC210800.024.7101061
CGCACGC121400.024.027831
TGCGACA102850.023.5501886
CCTAGCC221500.022.5757791
GTGGCGC176800.022.324073
TAGCCCT212250.022.0733953
TGGCGCA143250.021.6096634
GGCGATC43050.021.2957632
Produced by FastQC (version 0.11.2)