FastQCFastQC Report
Tue 8 Dec 2015
SLX-10776.A005.C80FNANXX.s_8.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-10776.A005.C80FNANXX.s_8.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29220820
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTT829050.283718937387794No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG819080.2803069865938054No Hit
CTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTATAA757130.25910634951380557No Hit
CGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGA757120.25910292729635925No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT581950.19915594428903777No Hit
ATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGCTTATTTGTAA518100.1773050858942357No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCATTGGGAT498520.17060438413432616No Hit
GTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCA498420.17057016195986288No Hit
GTACGTATATATTTTATTTAGATTTTATTCATAAATTAAGTTGAGAGCGC453120.15506751692799858No Hit
GTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTT415070.14204597954472187No Hit
CTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAA412190.1410603809201795No Hit
GTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAAT392920.13446576790110612No Hit
GTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTC355440.12163929691227009No Hit
CTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAAGG342310.11714592540524188No Hit
CGGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTA337690.11556486094503851No Hit
CTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGTAAATAGATAGAA303210.10376505519010075No Hit
CAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATAATCA298150.10203341316225897No Hit
ATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCTCCATAG295750.10121208097514034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA142500.039.658861
TCGTTTG297600.038.833852
TACGTAT152600.037.3016432
GTTTCGG307900.035.4293489
CGTTTGG321950.034.0315253
ACGTATA167250.033.7505073
GGTTTCG335550.032.5276268
CTCGTTT537600.030.8838941
CGTTGAT320400.030.0040841
CTCCGTT240900.028.8675041
GGAGTCT387200.028.573133
CGTATAT208500.028.1856064
GTGTACG104750.028.1114121
GAGTCTT401850.027.871664
CTCGTGT102600.027.5408761
AGTCTTG425700.026.7712335
GTTCGTT58600.026.5971551
CCTACGT212200.026.47572924-25
TACGTTC216800.025.91386226-27
TGGAGTC430350.025.6937892
Produced by FastQC (version 0.11.2)